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Leptospira DNA, Qualitative, Real-Time PCR
Test CodeLeptoQLQNI
Quest Code
17875
CPT Codes
87798
Preferred Specimen
5 mL frozen urine collected in a sterile, plastic, leak-proof container
Minimum Volume
1 mL urine • 0.8 mL whole blood • 0.5 mL CSF • 2 mL urine in aptima ®
Other Acceptable Specimens
1 mL whole blood collected in an EDTA (lavender-top) tube • 1 mL CSF collected in a sterile leak-proof container • 2 mL urine collected in Aptima ®
Instructions
Collect urine in a sterile, plastic container with a leak-proof cap. Freeze at -20° C immediately, and transport frozen. Do not thaw.
Transport Temperature
Urine: Frozen
Whole blood/CSF: Refrigerated (cold packs)
Whole blood/CSF: Refrigerated (cold packs)
Specimen Stability
Urine
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Whole blood/CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Urine in Aptima ®
Room temperature: 5 days
Refrigerated: 7 days
Frozen: 30 days
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Whole blood/CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Urine in Aptima ®
Room temperature: 5 days
Refrigerated: 7 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
FDA Status
LDT
Setup Schedule
Sets up 3 days a week.
Report Available
1-3 days
Reference Range
Not detected
Clinical Significance
PCR can be used to rapidly diagnose Leptospirosis, an infection caused by a waterborne spirochete of the genus Leptospira. In addition, testing of blood, CSF and urine may give an indication of the stage of infection.
Performing Laboratory
Quest Diagnostics Nichols Institute |
33608 Ortega Highway |
San Juan Capistrano, CA 92675-2042 |