Leptospira DNA, Qualitative, Real-Time PCR

Order ESOT

Test Code
Leptospira DNA, Qualitative, Real-Time PCR

Quest Code

CPT Codes

Preferred Specimen
5 mL frozen urine collected in a sterile, plastic, leak-proof container

Minimum Volume
1 mL urine • 0.8 mL whole blood • 0.5 mL CSF • 2 mL urine in Aptima®

Other Acceptable Specimens
1 mL whole blood collected in an EDTA (lavender-top) tube or CSF in sterile plastic leak-proof container • 2 mL urine in Aptima®

Collect urine in a sterile, plastic container with a leak-proof cap. Freeze at -20° C immediately, and transport frozen. Do not thaw.

Transport Temperature
Urine: Frozen
Whole blood/CSF: Refrigerated (cold packs)

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

Whole blood/CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Urine in Aptima ®
Room temperature: 5 days
Refrigerated: 7 days
Frozen: 30 days

Real-Time Polymerase Chain Reaction (RT-PCR)

FDA Status

Setup Schedule
Sets up 3 days a week.

Report Available
1-3 days

Reference Range
Not detected

Clinical Significance
PCR can be used to rapidly diagnose Leptospirosis, an infection caused by a waterborne spirochete of the genus Leptospira. In addition, testing of blood, CSF and urine may give an indication of the stage of infection.

Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.