BCR-ABL Tyrosine Kinase Inhibitor Resistance

Message

Performed at Mayo, Test Code BAKDM

Test Code

BATKIR

Alias/See Also

Test Code BAKDM
E255K
E355G
F317L
F369V
G250E
H396R
M244V
M351T
Q252H
T315I
Y253F
Y253H
T315A
V299L
Tyrosine kinase inhibitor (TKI)
Kinase domain mutation, ABL
Imatinib resistance
Chronic myeloid leukemia
Chronic myelogenous leukemia (CML)
BCR-ABL1
BCR/ABL
BCR ABL
Acute lymphoblastic leukemia (ALL)

CPT Codes
81170 (ZB1O2)

Preferred Specimen

Preferred:
Specimen Type: Whole blood
Container/Tube: EDTA (lavender top)
Specimen Volume: 3 mL

Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube.
3. Label specimen as blood.

Other Acceptable Specimens

Acceptable: Specimen Type: Bone marrow Container/Tube: EDTA (lavender top) Specimen Volume: 2 mL Collection Instructions: 1. Invert several times to mix bone marrow. 2. Send specimen in original tube. 3. Label specimen as bone marrow.

Instructions

The following information is required:
1. Patient's fusion type (p210, p190, p205 or p230)
2. Pertinent clinical history
3. Clinical or morphologic suspicion
4. Date of collection
5. Specimen source (blood or bone marrow)

https://www.mayomedicallaboratories.com/test-catalog/Overview/89609

Transport Temperature

Refrigerated specimens must arrive within 5 days (120 hours) of collection, and ambient specimens must arrive within 3 days (72 hours) of collection. Draw and package specimen as close to shipping time as possible.

Specimen Stability

Refrigerated 5 days
Ambient 72 hours

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis (mild and gross)
Moderately to severly clotted

Methodology
Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with Analysis of PCR Products by Sanger Sequencing

FDA Status
LDT

Setup Schedule

Monday-Friday

Report Available

5 days from setup

Performing Laboratory
Mayo Clinic Laboratories - Rochester Main Campus 200 First Street SW, Rochester, MN 55905

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.