HLA-B27 Antigen

Test Code

HB27

Quest Code

528

Alias/See Also

528

CPT Codes
86812<br /> **This test is not available for New York patient testing. For NY patient testing, use test code 93099.**

Preferred Specimen

5 mL whole blood collected in sodium heparin (green-top) tube

Minimum Volume

1 mL

Other Acceptable Specimens

EDTA (lavender-top) tube • ACD-A or ACD-B (yellow-top) tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 5 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Lithium heparin (green-top) tube • Clotted • Received frozen

Methodology
Flow Cytometry (FC)

Setup Schedule

Set up: Tues-Sun; Report available: 2-4 days

Reference Range

Negative

Clinical Significance

HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. Ankylosing spondylitis affects 1 in 1000 caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.