Atypical Pneumonia DNA Panel, Qualitative,  Real-Time PCR

Test Code
APNEUMOQLPCR


Quest Code
17610X


Alias/See Also
Atypical Pneumonia
PNEUMOPLQL
17610X


CPT Codes
87486, 87541, 87581, 87798

Includes
Mycoplasma pneumoniae DNA, Qualitative, Real-Time PCR
Legionella DNA, Qualitative, Real-Time PCR
Chlamydophila pneumoniae, DNA, Qualitative, Real-Time PCR


Preferred Specimen
1 mL sputum or bronchial lavage/wash collected in a sterile, leak-proof plastic container or throat or nasopharyngeal swab in M4 media or V-C-M medium (green-cap) tube or equivalent (UTM)


Minimum Volume
0.35 mL sputum or bronchial lavage/wash


Other Acceptable Specimens

CSF collected in a Sterile screw-cap container



Instructions
Nasopharyngeal and Throat swab in M4, VCM (UTM) Media (Use only sterile Dacron or rayon swabs.) Do NOT use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator sticks off near the tip to permit tightening of the cap.
Sputum: Collect in a sputum collection kit or a sterile, leak-proof plastic container.
Bronchial Lavage: Collect in a sterile, leak-proof plastic container.


Transport Temperature
Refrigerated (cold packs)


Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Calcium alginate swabs


Methodology
Real-Time Polymerase Chain Reaction

Setup Schedule
Sets up 7 days a week.


Reference Range
See individual tests


Clinical Significance
Atypical pneumonias share two features, which often makes it difficult to distinguish or diagnose the symptoms based on clinical presentation alone. The presentation is often a nonlobar, patchy, or interstitial pattern on chest x-ray. Routine culture and Gram stain fails to identify a causative agent. Respiratory virus can be grouped in this category, as well. Generally physicians will order this panel on severely ill and/or immunocompromised patients, not generally on out patients.


Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.