| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Pneumocystis jirovecii DNA, Qualitative Real-Time PCR
Test CodePJDQL(QNI)
Quest Code
18835
Alias/See Also
18835
CPT Codes
87594
Preferred Specimen
1 mL bronchoalveolar lavage, bronchial wash or sputum or
3 mm3 of tissue in a sterile leak-proof container
3 mm3 of tissue in a sterile leak-proof container
Minimum Volume
0.5 mL • 3 mm3
Transport Temperature
Refrigerated (cold-packs)
Specimen Stability
Room temperature: 24 hours
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction (PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Reference Range
Not detected
Clinical Significance
This test is intended for HIV-positive and immunocompromised individuals with clinical presentation and/or radiographic findings consistent with P. jirovecii infection. This assay can be used to aid in the diagnosis of P. jirovecii pneumonia (PCP), enabling the initiation of specific therapy. The PCR test has higher sensitivity and specificity when compared to alternative diagnostic methods such as staining (GMS, Calcofluor white, DFA), (1,3) β-D-glucan test or imaging studies. Due to the analytical sensitivity of this assay, the test can be positive in colonized individuals without clinical evidence of PCP. The results of this test should be interpreted in the context of pertinent clinical findings and history.
Reference
1. Bateman M, et al. Medical Mycology. 2020;58 :1015-1028.
Reference
1. Bateman M, et al. Medical Mycology. 2020;58 :1015-1028.
Performing Laboratory
| Quest Diagnostics Nichols Institute |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
