Wet Prep by NAH

Test Code

CPT Codes
87480, 87510, 87660

Preferred Specimen
Vaginal collected with AffirmTM VPIII Ambient Temperature Transport System (ATTS)

 Microbiology Specimen Collection Guide

  • For specimen collection, use only the Affirm Temperature Transport Media (ATTS).
    • Call Microbiology Department at 970-820-4156 to obtain AffirmTM VPIII Kit.
  • Open the seal on outer plastic pouch of the ATTS and remove all components.
  • Tear open the foil pouch and remove the ATTS Reagent Dropper. 
  • Break the ATTS Reagent Dropper by squeezing ampule, snap off the small grooved cap of the Sample Collection Tube and squeeze the contents of the dropper into the Sample Collection Tube. CAUTION: Break ampule close to its center one time only.  Do not manipulate dropper any further, as the plastic may puncture and injury may occur.
  • Peel wrapper to expose patient swab.  Remove Rayon swab and discard wrapper.
  • Collect patient specimen.
    • Place the patient in position for a pelvic examination.  Insert speculum into the vagina to permit visualization of the posterior vaginal fornix.
    • Using the sterile swab, obtain a specimen from the posterior vaginal fornix.  Twist or roll the swab against the vaginal wall two or three times, ensuring the entire circumference of the swab has touched the vaginal wall.  Swab the lateral vaginal wall while removing the swab.
    • Immediately place the patient swab in the Sample Collection Tube containing the ATTS Reagent.
    • Break swab shaft at pre-scored line just above the top of the tube.  Discard remaining shaft into an infectious waste container.
    • Place the Sample Collection Tube Cap over the exposed end of the swab and firmly press the cap onto the Sample Collection Tube. The Cap will snap onto the tube when it is properly seated.
    • Label each container with two patient identifiers (full name, DOB or MR), date and time of collection.
    • Maintain sterility and forward promptly.

Specimen Stability
Specimen Type Temperature Time
Vaginal in ATTS Ambient 72 hours
Vaginal in ATTS Refrigerated 72 hours

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Swab other than ATTS

Nucleic acid hybridization

Setup Schedule
Monday through Sunday; Continuously

Report Available
Same day

Reference Range
Negative for Candida, Gardnerella and Trichomonas (reported as positive or negative).

Performing Laboratory
Banner Fort Collins Medical Center Laboratory
McKee Medical Center Laboratory
North Colorado Medical Center Laboratory
Summit View Laboratory

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.