Hydroxyproline, Plasma

Test Code

HPPL

Quest Code

37558

Alias/See Also

37558

CPT Codes
83500

Preferred Specimen

2 mL plasma collected in a sodium heparin (green-top) tube

Patient Preparation
Collect plasma specimens after an overnight fast (or at least 4 hours after a meal).
Non-fasting samples are acceptable for pediatric patients.

Minimum Volume

0.25 mL

Other Acceptable Specimens

EDTA (lavender-top) tube • Lithium heparin (green-top) tube

Instructions

Plasma should be separated from cells as soon as possible after collection. Freeze plasma below -20° C.
Include date of collection and age of patient (age must be provided for age related reference ranges to print on the final report).

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Received room temperature

Methodology
Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Tues, Thurs-Fri; Report available: 5-9 days

Reference Range

≤30 days	13-72 umol/L
31 days-23 months	7-63 umol/L
2 years-17 years	6-32 umol/L
≥18 years	4-27 umol/L

Clinical Significance

Hydroxyproline is necessary for the construction of collagen. Defects in collagen synthesis lead to easy bruising, internal bleeding, breakdown of connective tissue of the ligaments and tendons, and increased risk to blood vessel damage. Increased loss of hydroxyproline in the urine is generally associated with breakdown of connective tissue due to disease process and may also be a manifestation of vitamin C deficiency.

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.