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Toxoplasma gondii DNA, Qualitative Real-Time PCR
MessageAvailable specimen types include: Amniotic fluid, Blood, CSF, and tissue.
Test Code
TOXOQLPCR
Quest Code
34451
Alias/See Also
34451
CPT Codes
87798
Preferred Specimen
1 mL amniotic fluid, CSF or 3 cubic mm tissue collected in a sterile, leak-proof container
Minimum Volume
3 cubic mm tissue • 0.2 mL vitreous fluid • 0.3 mL all other sample types
Other Acceptable Specimens
Serum • Whole blood or plasma collected in: EDTA (lavender-top) or ACD (yellow-top) tube • Amniotic fluid supernatant collected in: Sterile leak-proof container • 0.2 mL vitreous fluid collected in a sterile leak-proof container
Instructions
See link below for specimen details, methodology, setup schedule and reference ranges.
Transport Temperature
Frozen
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
All other samples
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
All other samples
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed whole blood
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1-2 days
Report Available
Maximum Laboratory Turn Around Time 3 days
Reference Range
Not Detected
Clinical Significance
Toxoplasma gondii, an obligate intracellular parasite, is an important opportunistic pathogen of immunosuppressed patients. In AIDS patients and transplant patients, this infection may result in a life-threatening encephalitis. T. gondii can also cause a fatal infection of the fetus if an infection is acquired during pregnancy. Fetal death or major abnormalities such as blindness and mental retardation may occur when infection is acquired during the first trimester. PCR methods may be useful in identifying T. gondii in CSF of immunosuppressed patients or in the amniotic fluid of mothers thought to be recently infected.
Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
33608 Ortega Highway |
San Juan Capistrano, CA 92675-2042 |
Additional Information
https://testdirectory.questdiagnostics.com/test/test-detail/34451/toxoplasma-gondii-dna-qualitative-real-time-pcr?p=r&q=34451&cc=MASTER