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Nonesterified Fatty Acids (Free Fatty Acids)
Test CodeFATTYACID
Quest Code
449
Alias/See Also
QNI 449
Fatty Acids
Fatty Acids
CPT Codes
82725
Preferred Specimen
1 mL frozen serum
Patient Preparation
Overnight fasting is preferred
Minimum Volume
0.3 mL
Other Acceptable Specimens
Serum collected in no additive (royal blue-top) tube • Plasma collected in: EDTA (lavender or royal blue-top) tube, 3.2% sodium citrate (light blue-top) tube, ACD solution B (yellow-top) tube, sodium fluoride or potassium oxalate (gray-top) tube, PTH-related protein (special collection tube), ACTH (lavender-top) tube, or PPT potassium EDTA (white-top) tube
Instructions
Serum or plasma should be removed from cells immediately after collection
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 2 hours
Frozen: 30 days
Refrigerated: 2 hours
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received thawed • Received refrigerated • Heparin plasma and patients on heparin therapy
Methodology
Spectrophotometry (SP) • Enzymatic
Setup Schedule
Night Sets up 2 days a week.
Reference Range
Newborns | <2.3 mmol/L |
1-12 Months | 0.50-1.60 mmol/L |
1-7 Years | 0.60-1.50 mmol/L |
8-17 Years | 0.20-1.10 mmol/L |
Adults | 0.07-0.88 mmol/L |
Clinical Significance
The Nonesterified Fatty Acids (Free Fatty Acids) assay is useful in evaluating lipolysis and fatty acid oxidation. Nonesterified Fatty Acids may be useful in evaluating patients with hypoglycemia and for nutritional assessment.
Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130