Platelet Antibody Screen (Direct) (5019)

Test Code

DIR PLT AB

Quest Code

140129P

Alias/See Also

5019
Plt Ab Screen (Direct)
Platelet Antibody, Direct, Flow Cytometry (5019)

CPT Codes
86023

Preferred Specimen

Whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

See instructions

Instructions

Do not refrigerate or freeze.
Effective 11/01/96, specimens submitted in ACD tubes (yellow-top) will no longer be accepted because of the high incidence of platelet clumping associated with this anticoagulant. If the platelet count is less than 20,000/ccm, submit 7-10 mL of whole blood.

Platelet Count	Minimum Volume
>=45,000	2 mL
20,000-45,000	5 mL
<20,000	10 mL

Pediatric minimum volume: The minimum volume is platelet count dependent. If possible, submit at least 1 mL EDTA blood.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 6 days
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Received frozen • ACD tubes

Methodology
Flow Cytometry (FC)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 3-4 days

Reference Range

Anti-IgG

Negative

Clinical Significance

Thrombocytopenia that is refractory to platelet transfusions may be due to direct platelet antibody. Testing is useful to differentiate immune from non-immune disorders.

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.