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Platelet Antibody Screen (Direct) (5019)
Test CodeDIR PLT AB
Quest Code
140129P
Alias/See Also
5019
Plt Ab Screen (Direct)
Platelet Antibody, Direct, Flow Cytometry (5019)
Plt Ab Screen (Direct)
Platelet Antibody, Direct, Flow Cytometry (5019)
CPT Codes
86023
Preferred Specimen
Whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
See Collection Instructions
Instructions
Do not refrigerate or freeze.
Effective 11/01/96, specimens submitted in ACD tubes (yellow-top) will no longer be accepted because of the high incidence of platelet clumping associated with this anticoagulant. If the platelet count is less than 20,000/ccm, submit 7-10 mL of whole blood.
Pediatric minimum volume: The minimum volume is platelet count dependent. If possible, submit at least 1 mL EDTA blood.
Effective 11/01/96, specimens submitted in ACD tubes (yellow-top) will no longer be accepted because of the high incidence of platelet clumping associated with this anticoagulant. If the platelet count is less than 20,000/ccm, submit 7-10 mL of whole blood.
Platelet Count | Minimum Volume |
≥45,000 | 2 mL |
20,000-45,000 | 4 mL |
≤20,000 | 10 mL |
Pediatric minimum volume: The minimum volume is platelet count dependent. If possible, submit at least 1 mL EDTA blood.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 6 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Received frozen • ACD tubes
Methodology
Flow Cytometry (FC)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 3-4 days
Reference Range
Anti-IgG | Negative |
Clinical Significance
Thrombocytopenia that is refractory to platelet transfusions may be due to direct platelet antibody. Testing is useful to differentiate immune from non-immune disorders.
Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042