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Plavix (P2Y12) Platelet Function Test

Message
Due to method testing constraints, this test must be drawn at North Colorado Medical Center.


Test Code
P2Y12 - NCMC


CPT Codes
85576

Preferred Specimen
Red top tube and Special Greiner Blue Top tube (see below)


Minimum Volume
2 mL in Red top
2 mL in each of Special Greiner Blue top tubes


Instructions
Venipuncture Collection
  1. Identify a site that is free of any peripheral venous infusions.
  2. Apply the tourniquet and identify the phlebotomy site.
  3. Release the tourniquet and cleanse the phlebotomy area.
  4. Reapply the tourniquet just before collection. It is important to minimize the amount of time that the tourniquet is applied prior to filling the tube in order to avoid activating the platelets while obtaining the sample.
  5. A 21 gauge needle or larger must be used to avoid hemolysis.
  6. Collect specimens in the following order: A Plain red top with at least 2 mL of whole blood must be drawn prior to drawing the Special Greiner Blue top tubes. Special Greiner Blue top tubes: If a single test is ordered: Collect 2 (Two) Special Greiner Blue top tubes. If both Aspirin Resistance Test and P2Y12 are ordered: Collect 3 (Three) Special Greiner Blue top tubes. (Note: the Special Greiner Blue top tube partially fills with 2.0 mL of blood, indicated by small black line. Do Not Overfill.) Note: If a CBC is to be collected at the same time, the CBC should be drawn last.
  7. Immediately gently invert the blue top tube 5 times. 
  8. Label the red and blue top tubes with Cerner labels. Write the collection time and collector ID on each tube. 
  9. All samples must be hand delivered to the lab (DO NOT SEND SAMPLES THROUGH THE TUBE SYSTEM).
  10. All samples must be kept at room temperature.
Central Line Collection:
  1. Ensure that the Central line is free of clots.
  2. Obtain a discard of at least 5 mL in order to clear the line.
  3. Obtain the sample for testing with a second syringe.
  4. Immediately transfer in the following order: 2.0 mL of blood into a plain red top tube Special Greiner Blue top tube: If a single test is ordered: Collect 2 (Two) Special Greiner Blue top tubes. If both Aspirin Resistance Test and P2Y12 are ordered: Collect 3 (Three) Special Greiner Blue top tubes (Note: the Special Greiner Blue top tube partially fills with 2.0 mL of blood, indicated by small black line. Do Not Overfill.)
  5. Immediately gently invert the blue top tube 5 times.
  6. Label the red and blue top tubes with Cerner labels. Write the collection time and collector ID on each tube.
  7. All samples must be hand delivered to the lab (DO NOT SEND SAMPLES THROUGH THE TUBE SYSTEM).
  8. All samples must be kept at room temperature.


Transport Temperature
Ambient


Specimen Stability
4 hours at room temperature


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Reject for any hemolysis, Insuffiicient volume, or tubed specimens.


Methodology
Turbidimetric-based optical detection.

Setup Schedule
Monday through Sunday; Continuously


Report Available
Same day


Reference Range
Off Drug Reference Range: 180 - 376 PRU
Values less than 180 PRU suggests evidence of a P2Y12 inhibitor effect.


Clinical Significance
The Verify Now PRU Test is a whole blood assay used in the laboratory to measure the level of platelet P2Y12 receptor blockade.


Performing Laboratory
North Colorado Medical Center Laboratory



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.