A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Cholesterol, High-Density Lipoprotein (HDL), Plasma or Serum
Test CodeHDL - NOCO
CPT Codes
83718
Includes
Note: This test is included in LIPIDP - Lipid Panel
Preferred Specimen
1 mL Plasma Green top (Lithium Heparin)
Minimum Volume
0.5 mL
Note: For neonate requirements see Neonate Minimum Blood Volumes
Note: For neonate requirements see Neonate Minimum Blood Volumes
Other Acceptable Specimens
1 mL serum from SST or Red Top
Instructions
- Fasting-overnight (12-14 hour).
- Patient must not consume any alcohol for 24 hours before specimen is drawn.
Specimen Stability
Temperature | Time |
Refrigerated | 5 days |
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis
Methodology
Colorometric Bichromatic Endpoint
Setup Schedule
Monday through Sunday; Continuously
Report Available
Same day
Reference Range
SV
Low HDL: <40 mg/dL
Normal HDL: 40 - 60 mg/dL
Desirable HDL: >60 mg/dL
MMC, BFCMC, & NCMC
MALE
0- 18 years: >45 mg/dL
18 - 150 years: >39
FEMALE
0 - 18 years: >45 mg/dL
18 - 150 years: >49 mg/dL
Low HDL: <40 mg/dL
Normal HDL: 40 - 60 mg/dL
Desirable HDL: >60 mg/dL
MMC, BFCMC, & NCMC
MALE
0- 18 years: >45 mg/dL
18 - 150 years: >39
FEMALE
0 - 18 years: >45 mg/dL
18 - 150 years: >49 mg/dL
Clinical Significance
High density lipoproteins (HDL) are responsible for the reverse transport of cholesterol from the peripheral cells to the liver. In the liver, cholesterol is transformed to bile acids which are then excreted into the intestine via the biliary tract. Monitoring of HDL-cholesterol in serum or plasma is of clinical relevance as the HDL-cholesterol concentration is important in the assessment of atherosclerotic risk. Elevated HDL-cholesterol concentrations protect against coronary heart disease (CHD), whereas reduced HDL-cholesterol concentrations, particularly in conjunction with elevated triglycerides, increase cardiovascular risk. A variety of methods are available to determine HDL-cholesterol, including ultracentrifugation (reference method in combination with cholesterol measurement by the Abell-Kendall method), electrophoresis, HPLC, precipitation, and direct methods. Of these, the direct methods are used routinely. Roche HDLC4 is also a direct method. The automated HDLC4 assay uses detergents, cholesterol esterase (CHER), cholesterol oxidase (CHOD) and peroxidase to form a colored pigment that is measured optically. The HDLC4 assay meets the 1998 National Institutes of Health (NIH) / National Cholesterol Education Program (NCEP) goals for precision and accuracy.
Performing Laboratory
Banner Fort Collins Medical Center Laboratory
Mckee Medical Center Laboratory
North Colorado Medical Center Laboratory
Summit View Laboratory