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Tropheryma whipplei (Whipple's) DNA, Qualitative, Real-Time PCR
Test CodeTROPWHIPPCRQL
Quest Code
11352X
CPT Codes
87798<br><strong>This test is not available for New York patient testing</strong>
Preferred Specimen
>3 mm3 fresh tissue submitted in a sterile, leak-proof container
Minimum Volume
3 mm3 tissue • 0.3 mL CSF, whole blood, or synovial fluid
Other Acceptable Specimens
0.7 mL CSF or synovial fluid submitted in a sterile, leak-proof container • 0.7 mL whole blood collected in an EDTA (lavender-top) tube
Transport Temperature
Frozen
Specimen Stability
Tissue and synovial fluid
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days
Whole blood and CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days
Whole blood and CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Reference Range
Not detected
Clinical Significance
Tropheryma whipplei (Whipple's disease) DNA, PCR is a highly sensitive and specific method to detect the presence of Tropheryma whipplei DNA in clinical specimens. The diagnosis of Tropheryma whipplei infection should not rely solely upon the result of a PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Tropheryma whipplei DNA at detectable levels in the samples tested and does not exclude the diagnosis of disease.
Performing Laboratory
Quest Diagnostics Nichols Institute |
33608 Ortega Highway |
San Juan Capistrano, CA 92675-2042 |