Parvovirus B19 DNA, Quantitative, Real-Time PCR

Test Code

PARVOPCRQT

Quest Code

19724

CPT Codes
87799

Preferred Specimen

1 mL plasma collected in an EDTA (lavender-top), ACD (yellow-top), or PPT (white-top) tube or
1 mL whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube or
1 mL amniotic fluid or amniotic fluid supernatant collected in a sterile leak-proof container or
1 mL serum

Minimum Volume

0.3 mL

Transport Temperature

Whole blood: Refrigerated (cold packs)
All other samples: Frozen

Specimen Stability

Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable

All other samples
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolyzed whole blood • Heparin plasma or whole blood

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Sets up 6 days a week.

Report Available

Reports in 1 to 2 days.

Reference Range

Parvovirus B19 DNA, QN PCR	Not Detected (copies/mL)
Parvovirus B19 DNA, QN PCR	Not Detected (Log copies/mL)

Clinical Significance

Parvovirus B19 is the cause of an infection known as "Fifth Disease". This infection affects children primarily and causes a rash on the face, trunk, and limbs. Joint pain and swelling is more common in adults. Although a portion of those affected have only mild disease, patients with sickle cell anemia, or similar types of chronic anemia, can suffer from severe acute anemia. Infection during pregnancy can lead to severe complications for the fetus. Infections in immunocompromised patients may lead to serious complications including nephropathy, myocarditis, and persistent anemia. Although serologic detection of circulating antibodies (Parvovirus B-19 IgM & IgG) is useful to determine recent exposure to the virus, DNA testing provides the most reliable evidence of a persistent infection. Quantitative results of Parvovirus B19 viral load may be useful in determining the response to therapy (usually IVIG) and may be beneficial in assessing the risk for nosocomial transmission.

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.