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Pneumocystis jirovecii (P. carinii), DFA
MessageOrder ESOT
Test Code
Pneumocystis jirovecii (P. carinii), DFA
Quest Code
34286
Alias/See Also
34286
CPT Codes
87281
Includes
Concentration
Preferred Specimen
3 mL bronchial lavage/wash, sputum, or bronchial alveolar lavage collected in a sterile leak-proof container
Minimum Volume
2 mL
Other Acceptable Specimens
Bronchial brushings collected on 2 acetone fixed slides
Instructions
Keep refrigerated.
All client prepared slides must be fixed in acetone for 10 minutes prior to drying. Client prepared slides made from tissue require director approval. Slides must be shipped in plastic or cardboard slide transport devices.
All client prepared slides must be fixed in acetone for 10 minutes prior to drying. Client prepared slides made from tissue require director approval. Slides must be shipped in plastic or cardboard slide transport devices.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Bronchial lavage/wash, sputum, bronchial alveolar lavage
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Slides
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 30 days
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Slides
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Broken slides • Slides fixed in formalin • Slides fixed in ethanol • Unfixed slides
Methodology
Direct Immunofluorescence Assay (DFA)
Setup Schedule
Set up: Mon-Sat; Report available: 2-4 days
Reference Range
Not detected
Clinical Significance
Pneumocystis carinii, now considered a fungus, is an opportunistic infection seen especially in patients infected with HIV and on immunosuppressive therapy, e.g., post-transplantation. Pneumocystis carinii causes pneumonia.
Performing Laboratory
| Quest Diagnostics Nichols Institute |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
