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Stool Pathogens Expanded, PCR
MessageStudies have shown that the efficacy of recovering enteric pathogens in stool specimens from hospitalized patients 3 days after admission is extremely low. Thus the standard of practice is to reject those specimens. If this is an exceptional case please contact the Microbiology Department 970-820-4156.
Test Code
STPCR EXPA - MM MMC
CPT Codes
87505, 87186 - MIC, per organism (if appropriate)
Includes
Vibrio Group (composed of V. cholerae and V. parahaemolyticus)
Yersinia enterocolitica
Norovirus GI/GII
Rotavirus A
Indicate on request form if isolation of other enteric pathogens is desired.
Yersinia enterocolitica
Norovirus GI/GII
Rotavirus A
Indicate on request form if isolation of other enteric pathogens is desired.
Preferred Specimen
Stool in Cary-Blair Vial - fill to line with stool.
Microbiology Specimen Collection Guide
Microbiology Specimen Collection Guide
Minimum Volume
Fresh Stool: 2 mL
Cary-Blair preserved specimen: fill to line
Cary-Blair preserved specimen: fill to line
Other Acceptable Specimens
Fresh Stool (unpreserved)
Instructions
A copy of the Stool Collection Patient Instructions (located under laboratory information) should be given to the outpatient or as follows:
- Submit fresh stool in a Para-PakTM culture and sensitivity preservative vial supplied.
- Add stool to the “fill to here” line on the vial.
- Carefully break up specimen using spoon.
- Tighten cap, and shake firmly to ensure that the specimen is adequately mixed. When mixing is complete, solution should appear homogeneous.
- Send specimen refrigerated.
- Label container (not the lid) with two patient identifiers (full name, DOB or MR) and date and time of collection.
- Forward promptly.
Specimen Stability
| Specimen Type | Temperature | Time |
| Fresh Stool | Refrigerate | 2 hours |
| Stool in Para-PakTM Vial | Refrigerate | 5 days |
Methodology
Polymerase Chain Reaction (PCR) Multiplex Verigene Enteric Pathogens Nucleic Acid Test
Setup Schedule
Monday through Sunday, Continuously
Reference Range
Vibrio: negative
Yersinia: negative
Norovirus: negative
Rotavirus: negative
Critical value (automatic call-back): positive result for any enteric pathogen
Yersinia: negative
Norovirus: negative
Rotavirus: negative
Critical value (automatic call-back): positive result for any enteric pathogen
Performing Laboratory
McKee Medical Center - Microbiology/Molecular Laboratory
