Dengue Fever Antibody (IgM)


Test Code

Quest Code

CPT Codes

Preferred Specimen
1 mL serum

Minimum Volume
0.25 mL

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Avoid using hemolyzed, lipemic or bacterial contaminated sera

Enzyme Immunoassay (EIA)

Setup Schedule

Set up: Sun, Tue, Thurs: Report available: 1-3 days

Reference Range

Interpretive Criteria
≤1.65 Antibody Not Detected
1.66-2.83 Equivocal
≥2.84 Antibody Detected
This assay detects IgM against all 4 Dengue virus serotypes, and the antibody response is not serotype-specific. A new IgM response may occur when exposure to a second dengue serotype occurs. In most patients, Dengue IgM is detectable after the sixth day following the onset of symptoms, and is highly suggestive of acute disease. Before day 6 of symptoms, both IgM and IgG may be negative and alternate testing (Dengue NS-1 Antigen or Dengue PCR) is recommended. A positive IgM may persist for several months after infection.
Cross-reactivity with other flaviviruses (eg. Zika virus) is known to occur; the extent and degree of serologic cross-reactivity varies. A positive Dengue virus IgM is presumptive evidence of infection. Patients at risk for Zika virus infection should also be evaluated for Zika virus per CDC guidelines.

Clinical Significance
Dengue hemorrhagic fever and Dengue shock syndrome are caused by infection of the RNA flavivirus transmitted by a mosquito vector. Paired acute and convalescent specimens that exhibit a significant change in titer are useful to confirm clinical diagnosis of infection.

Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.