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Protein S Antigen, Total and Free
Test CodePROTSAG
Quest Code
36457
Alias/See Also
36457
CPT Codes
85305, 85306
Preferred Specimen
2 mL frozen plasma collected in a 3.2% sodium citrate (light blue-top) tube
Minimum Volume
1 mL
Instructions
Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Freeze immediately and ship on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis
Methodology
Immunoturbidimetric
Setup Schedule
P.M. Sets up 2 days a week
Limitations
Protein S Antigen, Total: Expected impact by therapeutic levels (potential interference depends upon drug concentration): Warfarin: no effect to decrease; Heparin (UFH or LMWH): no effect; Dabigatran or Argatroban (Thrombin Inhibitors): no effect; Rivaroxaban or Apixaban (Factor Xa Inhibitors): no effect.
Protein S Antigen, Free: Expected impact by therapeutic levels (potential interference depends upon drug concentration): Warfarin: decrease; Heparin (UFH or LMWH): no effect; Dabigatran or Argatroban (Thrombin Inhibitors): no effect; Rivaroxaban or Apixaban (Factor Xa Inhibitors): no effect.
Protein S Antigen, Free: Expected impact by therapeutic levels (potential interference depends upon drug concentration): Warfarin: decrease; Heparin (UFH or LMWH): no effect; Dabigatran or Argatroban (Thrombin Inhibitors): no effect; Rivaroxaban or Apixaban (Factor Xa Inhibitors): no effect.
Reference Range
Protein S Antigen, Total | 70-140 % normal |
Protein S Antigen, Free | |
Male | 57-171 % normal |
Female | 50-147 % normal |
Clinical Significance
Antigen testing is appropriate when a functional activity deficiency is present. If low, Total Protein S Antigen assesses the Protein S deficiency as either Type I or III (IIa). Type II (IIb) Protein S deficiency has normal concentrations of Total and Free Protein S Antigen.
Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130