| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Herpes Simplex Virus, Type 1 and 2 DNA, Qualitative, Real-Time PCR
Test CodeHSVPCRQL
Quest Code
34257
Alias/See Also
34257
CPT Codes
87529 (x2)
Preferred Specimen
1 mL CSF, pleural fluid, pericardial fluid or amniotic fluid collected in a sterile leak proof container,
Serum collected in an SST® or sterile tubes with no anticoagulants,
Whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube,
Plasma collected in an EDTA (lavender-top) or ACD (yellow-top) or PPT tube,
Swab collected in a viral transport media, VCM or M4 or APTIMA® Unisex Swab Collection Kit
0.5 mL vitreous fluids collected in a sterile leak proof container
Minimum Volume
0.3 mL (0.35 mL BAL or bronchial washings)
Other Acceptable Specimens
Bronchoalveolar lavage or bronchial wash collected in a sterile leak proof container
Instructions
CSF: Collect in a sterile leak-proof container and store refrigerated or frozen.
Swab: Swab a lesion and place swab in a viral transport media, VCM or M4, or Aptima® Unisex Collection Kit (white label). Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST®) are recommended. Transfer serum into sterile plastic leak-proof container and store refrigerated or frozen.
Bronchial wash and BAL: Collect in a sterile leak proof container.
Swab: Swab a lesion and place swab in a viral transport media, VCM or M4, or Aptima® Unisex Collection Kit (white label). Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST®) are recommended. Transfer serum into sterile plastic leak-proof container and store refrigerated or frozen.
Bronchial wash and BAL: Collect in a sterile leak proof container.
Transport Temperature
Frozen
Specimen Stability
All other specimens:
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (whole blood unacceptable)
APTIMA® Unisex Swab collection:
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (whole blood unacceptable)
APTIMA® Unisex Swab collection:
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
A.M. Sets up 7 days a week.
Report Available
1-2 days from receipt at ref. lab
Reference Range
| HSV 1 DNA | Not detected |
| HSV 2 DNA | Not detected |
Clinical Significance
DNA testing is analytically more sensitive than culture, especially in patients with encephalitis or meningitis. DNA testing may be useful in diagnosis of infection in neonates. Neonates who have been exposed to HSV can develop disseminated infection and encephalitis. Encephalitis is usually due to HSV I whereas meningitis is usually due to HSV II. DNA testing provides reliable means to define the type.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
