ACTH, Plasma

Test Code

ACTH

Quest Code

211

Alias/See Also

Adrenocorticotropic Hormone

CPT Codes
82024

Preferred Specimen

1.5 mL plasma collected in an EDTA (lavender-top) tube

Patient Preparation
Draw specimens between 7AM and 10AM. If drawn during any other time, the reference ranges do not apply.

Minimum Volume

0.5 mL

Other Acceptable Specimens

PPT-potassium EDTA (white-top) tube

Instructions

Centrifuge immediately after collection to separate plasma from cells within 2 hours from collection. Transfer plasma to a plastic specimen transport container and mark the specimen type as plasma on the container. Freeze immediately.

Transport Temperature

Frozen

Specimen Stability

Room temperature: 16 hours
Refrigerated: 24 hours
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received thawed • Received room temperature • Received refrigerated

Methodology
Immunoassay (IA)

Setup Schedule

Set up: Tues-Sat; Report available: 1-2 days

Report Available

5 days

Limitations

With patients receiving more than 5mg/day of biotin, a sample should be taken no sooner than eight hours after previous dose. Patients treated with monoclonal mouse antibodies may have inaccurate results. High titer of antibodies such as streptavidin and ruthenium may cause interference. Due to its correlation with cortisol levels, ACTH concentration is affected by stress. Any single measure may be within the reference range for patients with increased production (Cushing's disease) or minimal production of ACTH.

Reference Range

<3 Years	Not established
3-17 Years	9-57 pg/mL
≥18 Years	6-50 pg/mL

Clinical Significance

Determination of ACTH is useful in differentiating between primary and secondary adrenocortical hypo- and hyperfunctional disorders: Addison's Disease, Cushing's Syndrome, Adrenal Carcinoma, Ectopic ACTH Syndrome, Nodular Hyperplasia.

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.