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Fungus Culture
MessageFor Blood Culture for Fungus (Mould) refer to C BLF
Calcofluor white stains are automatically performed for the following sources, when a fungus culture is ordered:
Calcofluor white stains are automatically performed for the following sources, when a fungus culture is ordered:
- Bronchoalveolar Lavage
- Corneal scraping
- Liquid specimens such as abscess fluids, eye fluids
- Sterile body fluid
- Tissue
Test Code
C F
Alias/See Also
Culture for Aspergillus, Cocci, Coccidioides species, Coccidioides posadasii, Coccidioides immitis, Histoplasma, Dermatophytes or Nocardia
Preferred Specimen
Bone Marrow: submit in an Isolator Tube or SPS tube. Other specimens submit in sterile container (examples include skin and nail scrapings)
Patient Preparation
To Collect/ Submit Skin Scrapings: Clean area with 70% alcohol and scrape off skin at active border; include some healthy skin. Place in a sterile screw-capped container.
To Collect/ Submit Nail Scrapings: Clean area with 70% alcohol and scrape with a sterile scalpel; discard first portion; send deeper scrapings for culture.
Place as much material as possible in a sterile screw-capped container.
Minimum Volume
Bone Marrow submitted in a pediatric isolator tube MUST have a minimum of 0.5 ml in a 1.5 ml tube
Instructions
*Call Laboratory for Isolator Tube(s)
Describe specific source with detail, such as: Sputum, expectorated Sputum, induced Bronch wash, LLL Pleural fluid Abscess left leg Skin Scrapings Nail Scrapings
Describe specific source with detail, such as: Sputum, expectorated Sputum, induced Bronch wash, LLL Pleural fluid Abscess left leg Skin Scrapings Nail Scrapings
Transport Temperature
Refrigerated
Specimen Stability
For Fluid or Tissue: Send to the Lab within 30 minutes of collection. If specimen is received after 24 hours the specimen will be rejected and the culture cancelled.
For eSwabs: Send to the Lab within 2 hours of collection. If specimen is received after 48 hours the specimen will be rejected and the culture cancelled.
For eSwabs: Send to the Lab within 2 hours of collection. If specimen is received after 48 hours the specimen will be rejected and the culture cancelled.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Non-sterile, Insufficient volume, Leaking containers Specimen received in VTM or preservative such as formalin or cytolyte Dry swabs Specimens with prolonged transport time (greater than 24 hours)
Clinical Significance
Fungi can infect the cerebral spinal fluid, other body fluids, and tissues. Infections occur more commonly among patients who are immunocompromised. Early diagnosis and effective treatment can reduce the likelihood of severe sequelae.