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Phosphatidylserine/Prothrombin (PS/PT) Antibodies (IgG, IgM)
Test CodePHOSERPT
Quest Code
11447
CPT Codes
83516 (x2)
Preferred Specimen
1 mL platelet-poor plasma collected in a sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
Other Acceptable Specimens
Serum
Instructions
Platelet-poor plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a platic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Serum: Completely fill the Vacutainer and allow the blood to clot in an upright position for at least 30 minutes, but no longer than 1 hour before centrifugation. Transfer the serum to a plastic transport tube.
Serum: Completely fill the Vacutainer and allow the blood to clot in an upright position for at least 30 minutes, but no longer than 1 hour before centrifugation. Transfer the serum to a plastic transport tube.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: 30 days
Frozen: 30 days
Refrigerated: 30 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic
Methodology
Immunoassay (IA)
Setup Schedule
A.M. Sets up 3 days a week.
Report Available
Reports in 1 to 3 days.
Reference Range
Phosphatidylserine/Prothrombin Ab (IgG) | ≤30 U |
Phosphatidylserine/Prothrombin Ab (IgM) | ≤30 U |
Clinical Significance
Antiphospholipid Syndrome (APS) is an acquired autoimmune thrombophilia characterized by thrombosis (venous and/or arterial or microvascular) and/or pregnancy loss or morbidity in association with persistent positivity of a heterogenous group of autoantibodies known as anti-phospholipid antibodies (APL).
The international laboratory consensus criteria for the diagnosis of APS includes a positive lupus anticoagulant and/or moderate to high positivity for IgG/IgM Cardiolipin and Beta-2-Glycoprotein I antibodies. Although not included in the criteria classification for APS diagnosis, antibodies directed against a phosphatidylserine/prothrombin complex (PS/PT) have been reported to have a high association with venous thrombosis and obstetric abnormalities, but not with arterial thrombosis. In addition, a very high degree of concordance between the concentration of aPS/PT and lupus anticoagulant activity has been demonstrated.
The international laboratory consensus criteria for the diagnosis of APS includes a positive lupus anticoagulant and/or moderate to high positivity for IgG/IgM Cardiolipin and Beta-2-Glycoprotein I antibodies. Although not included in the criteria classification for APS diagnosis, antibodies directed against a phosphatidylserine/prothrombin complex (PS/PT) have been reported to have a high association with venous thrombosis and obstetric abnormalities, but not with arterial thrombosis. In addition, a very high degree of concordance between the concentration of aPS/PT and lupus anticoagulant activity has been demonstrated.
Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042