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Tissue Culture
MessageAnaerobic culture of tissue or biopsy refer to C TISAN
AFB (Mycobacterium, TB, MAC, MAI, atypical Mycobacterium) culture (includes AFB smear) refer to C AFB
Tissue for fungus culture (mould)- includes calcofluor direct exam for fungal elements refer to C F
Respiratory tissue for Legionella spp refer to C LEG
Respiratory tissue for Pneumocystis refer to PCFA
Helicobacter pylori culture available as a sendout test to QDNI
AFB (Mycobacterium, TB, MAC, MAI, atypical Mycobacterium) culture (includes AFB smear) refer to C AFB
Tissue for fungus culture (mould)- includes calcofluor direct exam for fungal elements refer to C F
Respiratory tissue for Legionella spp refer to C LEG
Respiratory tissue for Pneumocystis refer to PCFA
Helicobacter pylori culture available as a sendout test to QDNI
Test Code
C TIS
Includes
Routine culture of tissue/biopsy includes Gram stain
Preferred Specimen
Collect as much sample as possible, transfer aseptically to a sterile, screw-capped container or anaerobic collection tube.
NOTE: For biopsy specimens please transport in a small amount of liquid media (sterile saline) to keep moist. Do Not allow the specimen to dry out.
NOTE: For biopsy specimens please transport in a small amount of liquid media (sterile saline) to keep moist. Do Not allow the specimen to dry out.
Instructions
Describe exact source of tissue such as:
Lung tissue, left lower lobe
Tissue, right leg
Tissue, esophageal biopsy
Brain biopsy
Do not allow sample to dry out. Add a tiny amount of sterile saline if necessary.
Lung tissue, left lower lobe
Tissue, right leg
Tissue, esophageal biopsy
Brain biopsy
Do not allow sample to dry out. Add a tiny amount of sterile saline if necessary.
Transport Temperature
Ambient/ Room Temperature
Specimen Stability
Transport all specimens at ambient/ room temperature.
Send to the Lab within 30 minutes of collection. Specimen should be received in lab in <24 hours.
Store at Ambient/ Room Temperature
Send to the Lab within 30 minutes of collection. Specimen should be received in lab in <24 hours.
Store at Ambient/ Room Temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unacceptable specimens:
Tissue received in formalin or other preservative
Excessive transport time (>24 hours). If specimen is received after 24 hours, specimen will be rejected and test cancelled.
Tissue received in formalin or other preservative
Excessive transport time (>24 hours). If specimen is received after 24 hours, specimen will be rejected and test cancelled.
Report Available
The Gram stain report will be available <24 hours.
A preliminary culture report will be available <24 hours.
A final negative culture report will be generated <5 days.
All positive cultures will be reported immediately.
A preliminary culture report will be available <24 hours.
A final negative culture report will be generated <5 days.
All positive cultures will be reported immediately.
Clinical Significance
Aerobic/Anaerobic bacteria cause a variety of human infections. Proper specimen collection and transport, media and incubation are important criteria for the recovery of organisms. The primary aerobic bacterial agents for skin and tissue infections include Staphylococcus aureus, coagulase-negative staphylococci, Enterococci, members of the Enterobacterales, Pseudomonas aeruginosa, and beta-hemolytic streptococci. The Gram stain is a critical test for the rapid, presumptive diagnosis of infectious agents and also serves to assess the quality of clinical specimens. The results of aerobic/anaerobic cultures assist the clinician with diagnosis and treatment of patients with bacterial infections. Proper interpretation of culture results is dependent on specimen source and known pathogenicity of the isolated organism.
