Antiphospholipid Syndrome Diagnostic Panel

Message

Must be collected only at the following locations:
Duke Street Lab Testing
Suburban Pavilion Lab Testing
Women and Babies' Hospital Lab Testing

Test Code

19872

Alias/See Also

ASYND
LAB0001867

CPT Codes
85613, 85730, 86146 (x3), 86147 (x3)

Includes

Cardiolipin Antibodies (IgA, IgG, IgM)
Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM)
Lupus Anticoagulant Evaluation with Reflex (PTT-LA and dRVVT with Reflex Confirmations)

If PTT-LA Screen is prolonged (>40 seconds), then Hexagonal Phase Confirm is performed at an additional charge (CPT code(s): 85598).
If Hexagonal Phase Confirm is positive or weakly positive, then Thrombin Clotting Time will be performed at an additional charge (CPT code(s): 85670).
If dRVVT Screen is prolonged (>45 seconds), then dRVVT Confirmation will be performed at an additional charge (CPT code(s): 85597).
If dRVVT Confirm is positive, then dRVVT 1:1 Mix will be performed at an additional charge (CPT code(s): 85613).

Preferred Specimen

1 mL frozen platelet-poor plasma collected in each of three separate 3.2% sodium citrate (light blue-top) tubes

Minimum Volume

1 mL (x2)

Instructions

Plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.

Transport Container

Plastic vials

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Grossly lipemic • Received room temperature • Received refrigerated • Serum

Methodology
Immunoassay (IA) • Photo-Optical Clot Detection

Setup Schedule

Set up and Report available: See individual tests

Reference Range

See individual tests

Clinical Significance

This assay may be useful in supporting or ruling out a diagnosis of antiphospholipid syndrome.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.