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HLA-B27 Antigen (NY)
Test Code93099
CPT Codes
86812<br><strong>This test is for New York patient testing only. For non-New York patients, use test code 528.</strong>
Preferred Specimen
5 mL whole blood collected in a sodium heparin (green-top) tube
Minimum Volume
1 mL
Other Acceptable Specimens
Whole blood collected in an EDTA (lavender-top), or acid citrate dextrose ACD-A or B (yellow-top) tube
Transport Temperature
Room temperature
Specimen Stability
Sodium heparin (green-top) tube
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
EDTA (lavender-top) tube
Room temperature: 30 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
ACD-A or ACD-B (yellow-top) tube
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
EDTA (lavender-top) tube
Room temperature: 30 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
ACD-A or ACD-B (yellow-top) tube
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lithium heparin (green-top) tube • Clotted • Received frozen
Methodology
Flow Cytometry (FC)
Setup Schedule
Set up: Mon-Sat; Report available: Next day
Reference Range
Negative
Clinical Significance
HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's Disease. Ankylosing spondylitis affects 1 in 1000 Caucasians. Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153