Herpes Simplex Virus, Type 1 and 2 DNA, Quantitative, Real-Time PCR

Test Code

19502

CPT Codes
87530 (x2)

Preferred Specimen

1 mL CSF, vitreous, pericardial, pleural, or amniotic fluid collected in a sterile, leak-proof container, or
1 mL plasma or whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube, or
1 mL serum

Minimum Volume

0.3 mL

Other Acceptable Specimens

Bronchoalveolar lavage or bronchial wash collected in a sterile, leak-proof container

Transport Temperature

Whole Blood: Refrigerated (cold packs)
All other specimens: Frozen

Specimen Stability

Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable

All other specimen types
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (glass tubes unacceptable)

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 1 day

Reference Range

HSV 1, DNA, QN, PCR	Not Detected
HSV 2, DNA, QN, PCR	Not Detected

Clinical Significance

The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 100-2,000,000 copies/mL.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.