A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Herpes Simplex Virus, Type 1 and 2 DNA, Quantitative, Real-Time PCR
Test Code19502
CPT Codes
87530 (x2)
Preferred Specimen
1 mL CSF, vitreous, pericardial, pleural, or amniotic fluid collected in a sterile, leak-proof container, or
1 mL plasma or whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube, or
1 mL serum
1 mL plasma or whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube, or
1 mL serum
Minimum Volume
0.3 mL
Other Acceptable Specimens
Bronchoalveolar lavage or bronchial wash collected in a sterile, leak-proof container
Transport Temperature
Whole Blood: Refrigerated (cold packs)
All other specimens: Frozen
All other specimens: Frozen
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
All other specimen types
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (glass tubes unacceptable)
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
All other specimen types
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (glass tubes unacceptable)
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1 day
Reference Range
HSV 1, DNA, QN, PCR | Not Detected |
HSV 2, DNA, QN, PCR | Not Detected |
Clinical Significance
The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 100-2,000,000 copies/mL.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153