| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Antimicrobial Susceptibility, Aerobic Bacteria, MIC
Test Code94881
CPT Codes
87186
Preferred Specimen
Bacterial isolate swab collected with E-Swab collection and transport system
Note: Organism identification and specimen source (anatomical body site) are required.
Note: Organism identification and specimen source (anatomical body site) are required.
Other Acceptable Specimens
Flocked swab in 1 mL liquid Amies transport medium in 12 x 80 mm tube or
Pure culture of organism from source cultured on Agar slant or other appropriate media
Pure culture of organism from source cultured on Agar slant or other appropriate media
Instructions
E-Swab or flocked swab:
1. Perform isolation of bacterial isolate.
2. Utilize the flocked swab to obtain an adequate sample of pure cultured isolate. Do not submit mixed cultures.
3. Place swab into the transport system containing 1 mL liquid Amies transport medium.
4. If needed, break off end of swab and close the transport tube.
5. Place the transport system into the secondary infectious container for shipment.
6. Each isolate must be submitted under a separate order.
Note: For the following organisms, submit an agar slant or other appropriate media to ensure viability upon arrival to the laboratory; Neisseria gonorrhoeae, Campylobacter species, Helicobacter pylori, and any other fastidious organism.
Pure culture:
1. Perform isolation of bacterial isolate.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
3. Place the agar slant or other appropriate media into the secondary infectious container for shipment.
4. Each isolate must be submitted under a separate order.
1. Perform isolation of bacterial isolate.
2. Utilize the flocked swab to obtain an adequate sample of pure cultured isolate. Do not submit mixed cultures.
3. Place swab into the transport system containing 1 mL liquid Amies transport medium.
4. If needed, break off end of swab and close the transport tube.
5. Place the transport system into the secondary infectious container for shipment.
6. Each isolate must be submitted under a separate order.
Note: For the following organisms, submit an agar slant or other appropriate media to ensure viability upon arrival to the laboratory; Neisseria gonorrhoeae, Campylobacter species, Helicobacter pylori, and any other fastidious organism.
Pure culture:
1. Perform isolation of bacterial isolate.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
3. Place the agar slant or other appropriate media into the secondary infectious container for shipment.
4. Each isolate must be submitted under a separate order.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Determined by viability
Refrigerated: Determined by viability
Frozen: Unacceptable
Refrigerated: Determined by viability
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Agar plate
Methodology
Minimum Inhibitory Concentration (Agar Dilution or Broth Microdilution or Gradient Diffusion), or Disk Diffusion (if appropriate)
Setup Schedule
Set up: Daily; Report available: 4-7 days
Reference Range
See Laboratory Report
Clinical Significance
Determining the in vitro susceptibility of aerobic bacteria involved in human infections.
Performing Laboratory
Mayo Clinic Laboratories
200 First Street SW
Rochester, MN 55905
