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Fecal Leukocyte Stain [3930X]
Test Code562
CPT Codes
89055
Preferred Specimen
10 grams or 10 mL stool or fecal pus collected in a Total-Fix® transport vial
Patient Preparation
Patients should refrain from ingesting barium for 7 days before specimen collection
Minimum Volume
5 grams or 5 mL
Other Acceptable Specimens
Stool or fecal pus collected in Zn-PVA fixative transport vial
Instructions
1. The specimen must be passed into a clean, dry container and must not be contaminated with urine or water. If a toilet is used, the water supply may be cut off and the bowl drained.
2. Add stool to bring the liquid level to the "Fill to here" line on the Total-Fix or Zn-PVA transport vial. Mix contents thoroughly until homogeneous.
2. Add stool to bring the liquid level to the "Fill to here" line on the Total-Fix or Zn-PVA transport vial. Mix contents thoroughly until homogeneous.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unpreserved stool • Stools preserved in transport media other than Total-Fix or Zn-PVA
Methodology
Microscopy
Setup Schedule
Set up: Daily; Report available: Next day
Limitations
In 10-15% of patients with invasic bacterial infection, their fecal stool will not reveal leukocytes. Fecal leukocytes may be present in idiopathic inflammatory bowel disease.
Reference Range
See Laboratory Report
Clinical Significance
The presence of leukocytes is an indicator of inflammation. Generally, inflammation is a product of bacteria-host interaction.
Performing Laboratory
Quest Diagnostics Nichols Institute |
14225 Newbrook Drive |
Chantilly, VA 20153 |