| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Mycobacterium avium-intracellulare DNA, Qualitative, Real-Time PCR
Test Code16064
CPT Codes
87561 (x2)
Includes
M. avium DNA and M. intracellulare DNA
Preferred Specimen
2 mL sputum collected in a sterile, leak-proof container
Minimum Volume
1 mL • 2 cubic mm tissue
Other Acceptable Specimens
Whole blood collected in: EDTA (lavender-top) tube, or PPT (white-top) tube • Bronchial lavage/wash • Pleural fluid • 3 cubic mm tissue submitted in sterile plastic leak-proof container
Instructions
Samples must be kept refrigerated after collection and during shipment.
Sputum: Collect in a sputum collection kit or a sterile, plastic leakproof container.
BAL, Bronch wash, pleural fluid, or tissue: Collect in a sterile, plastic leakproof container.
Whole blood: Collect blood in sterile tubes containing EDTA anticoagulants (lavender-top) or PPT (white-top) tube.
Sputum: Collect in a sputum collection kit or a sterile, plastic leakproof container.
BAL, Bronch wash, pleural fluid, or tissue: Collect in a sterile, plastic leakproof container.
Whole blood: Collect blood in sterile tubes containing EDTA anticoagulants (lavender-top) or PPT (white-top) tube.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Sputum, BAL, Bronch wash, Pleural fuild, Whole blood
Room temperature: 48 hours
Refrigerated: 10 days
Frozen: 30 days
Tissue
Room temperature: 48 hours
Refrigerated: 5 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 10 days
Frozen: 30 days
Tissue
Room temperature: 48 hours
Refrigerated: 5 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens containing heparin
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Tues, Fri; Report available: 3-5 days
Reference Range
| M. avium DNA | Not detected |
| M. intracellulare DNA | Not detected |
Clinical Significance
This test is used to detect the presence of either M. avium or M. intracellulare in a patient's specimen. The use of Real-Time PCR to assay for the presence of M. avium and/or M. intracellulare DNA in clinical specimens allows for rapid patient testing (3 to 4 hours compared to several weeks or more for conventional culture and DNA hybridization) and for distinguishing infections caused by M. tuberculosis or other mycobacterial species.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
