HLA-B*58:01 Typing

Test Code

93932

CPT Codes
81381

Preferred Specimen

5 mL whole blood collected in an EDTA (lavender, pink, or royal blue-top), sodium heparin (green-top), or 3.2% sodium citrate (light blue-top) tube

Minimum Volume

3 mL

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 10 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Clotted sample

Methodology
Screen: Allele Specific PCR • Confirmation: LABType® SSO

Setup Schedule

Set up: Tues, Thurs, Sat; Report available: 7 days

Reference Range

Negative

Clinical Significance

HLA B*58:01 has been associated with severe cutaneous adverse reactions (SCAR) in response to the drug Allopurinol. The Clinical Pharmacogenomic Implementation Consortium, CPIC, has published an article recommending genotyping prior to getting allopurinol treatment, and recommended that the drug should not be administered to patients with the HLA-B*58:01 allele. Frequency of allele varies with ethnicity: 6% to 7% in Asians, 1% in Caucasians. Similarly, the American College of Rheumatology has published guidelines for the management of gout utilizing allopurinol among other medications. They recommend HLA-B*58:01 testing in high-risk all patients of Han Chinese and Thai descent and Koreans with stage 3 or worse chronic kidney disease.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.