Borrelia miyamotoi DNA, Real-Time PCR, Miscellaneous

Test Code

93795

CPT Codes
87478

Preferred Specimen

1 mL CSF, or synovial fluid collected in a sterile leak-proof container, or
1 mL whole blood collected in an EDTA (lavender-top) tube, or ACD (yellow-top) tube

Minimum Volume

0.3 mL

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

CSF, Synovial fluid
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Whole blood containing heparin as an anticoagulant

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Sat; Report available: 1-3 days

Reference Range

Not detected

Clinical Significance

Persons infected with Borrelia miyamotoi most likely have fever, joint pains, fatigue, and headache, though unlike Lyme disease, rash is uncommon.
The diagnosis of Borrelia miyamotoi is most often made by clinical examination combined with evidence of tick bite (or known or presumed exposure to ticks in endemic areas). Amplification of B. miyamotoi genomic DNA from blood, fluids or tissues confirms the diagnosis.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.