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Borrelia miyamotoi DNA, Real-Time PCR, Miscellaneous
Test Code93795
CPT Codes
87478
Preferred Specimen
1 mL CSF, or synovial fluid collected in a sterile leak-proof container, or
1 mL whole blood collected in an EDTA (lavender-top) tube, or ACD (yellow-top) tube
1 mL whole blood collected in an EDTA (lavender-top) tube, or ACD (yellow-top) tube
Minimum Volume
0.3 mL
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
CSF, Synovial fluid
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Whole blood containing heparin as an anticoagulant
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 1-3 days
Reference Range
Not detected
Clinical Significance
Persons infected with Borrelia miyamotoi most likely have fever, joint pains, fatigue, and headache, though unlike Lyme disease, rash is uncommon.
The diagnosis of Borrelia miyamotoi is most often made by clinical examination combined with evidence of tick bite (or known or presumed exposure to ticks in endemic areas). Amplification of B. miyamotoi genomic DNA from blood, fluids or tissues confirms the diagnosis.
The diagnosis of Borrelia miyamotoi is most often made by clinical examination combined with evidence of tick bite (or known or presumed exposure to ticks in endemic areas). Amplification of B. miyamotoi genomic DNA from blood, fluids or tissues confirms the diagnosis.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153