A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Lupus Panel 4 with ANA, IFA with Reflex to Titer and Pattern
Test CodeCPT Codes
83516, 86038, 86160 (x2), 86225, 86235 (x5), 86376, 86431
Includes
Complement Component C3c and C4c
DNA (ds) Antibody
Ribosomal P Antibody
Sm Antibody
Sm/RNP Antibody
Sjogren’s Antibodies (SS-A, SS-B)
Thyroid Peroxidase Antibody (TPO)
Scleroderma Antibody (Scl-70)
Rheumatoid Factor
If the ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).
Preferred Specimen
Minimum Volume
Transport Temperature
Specimen Stability
Refrigerated: 4 days
Frozen: 8 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Methodology
Immunoassay (IA) • Immunoturbidimetric Assay
Setup Schedule
Reference Range
Clinical Significance
This panel can be helpful in the diagnosis of systemic lupus erythematosus (SLE) and several other autoimmune diseases, including mixed connective tissue disease, Sjogren syndrome, systemic sclerosis, and autoimmune thyroid disease. The panel simultaneously tests for antinuclear antibodies (ANAs) with an immunofluorescence assay (IFA), performed on human epithelial type 2 (HEp-2) cells, and 11 biomarkers associated with these conditions. For specimens with positive ANA IFA results, reflex testing for ANA titer and pattern is performed.
The ANA testing has high sensitivity (97%) for SLE but limited specificity (34%) [1]. Thus, a positive ANA test does not exclude other autoimmune diseases with similar clinical features [2]. Testing for specific antibodies in a panel may expedite the evaluation of SLE but is generally not recommended [3].
The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.
References
1. Petri M, et al. Arthritis Rheum. 2012;64(8):2677-2686.
2. Kavanaugh A, et al. Arch Pathol Lab Med. 2000;124(1):71-81.
3. Yazdany J, et al. Arthritis Care Res (Hoboken). 2013;65(3):329-339.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153