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Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation
Test Code91981
CPT Codes
86353
Preferred Specimen
5 mL whole blood collected in a sodium heparin (green-top) tube
Minimum Volume
3 mL
Instructions
Whole blood must be transported at room temperature and delivered to the testing laboratory within 48 hours after collection.
Maintain and transport blood at room temperature. Avoid temperatures <15° C and >37° C. In hot weather, it may be necessary to pack the specimen in a container with insulating material around it and place this container inside another one that contains a cold pack (ice pack) and absorbent material. This method will help retain the specimen at ambient temperature. For longitudinal studies, draw specimens at the same time of day to minimize diurnal variation.
Clients: Contact the lab prior to ordering for special logistics arrangements.
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions.
Submit Monday-Thursday only. Do not draw day before a holiday. Samples received in testing lab >48 hours after collection will be rejected. Date and time of draw are required and must be provided.
Maintain and transport blood at room temperature. Avoid temperatures <15° C and >37° C. In hot weather, it may be necessary to pack the specimen in a container with insulating material around it and place this container inside another one that contains a cold pack (ice pack) and absorbent material. This method will help retain the specimen at ambient temperature. For longitudinal studies, draw specimens at the same time of day to minimize diurnal variation.
Clients: Contact the lab prior to ordering for special logistics arrangements.
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions.
Submit Monday-Thursday only. Do not draw day before a holiday. Samples received in testing lab >48 hours after collection will be rejected. Date and time of draw are required and must be provided.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received refrigerated • Received frozen • Clotted specimens • Samples collected in lithium heparin tubes
Methodology
Cell Culture (CC) • Scintillation Counter
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Tues-Fri; Report available: 15 days
Reference Range
| PWM, CPM | 29,100-125,000 Net CPM |
| PWM, SI | Not established |
| Cell Viability | 80-100% |
Clinical Significance
Measurement of human lymphocytes' proliferative responses to various stimuli is a fundamental technique used to assess their biological status and functions. Mitogens, such as plant lectins phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), are able to nonspecifically stimulate lymphocyte proliferation and used to evaluate patient immune responsiveness.
Performing Laboratory
Quest Diagnostics Nichols Inst San Juan Capistrano
33608 Ortega Highway
San Juan Capistrano, CA 92675
