Human Transforming Growth Factor beta 1 (TGF-b1)

Test Code
91238


CPT Codes
83520

Preferred Specimen
1 mL platelet-free frozen plasma collected in a EDTA (lavender-top) tube - See instructions


Minimum Volume
0.25 mL


Instructions

Collect in lavender-top tube using EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Centrifuge plasma again at 3000 x g for 10 minutes for complete platelet removal. Freeze at -20°C or below.

For fixed speed centrifuges such as 645e:
Collect in lavender-top tube using EDTA as an anticoagulant. Centrifuge 3 times for 10 minutes at 1600 x g while decanting the plasma each time before the next spin within 30 minutes of collection. Freeze at -20°C or below.



Transport Container
Plastic screw-cap vial


Transport Temperature
Frozen


Specimen Stability
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen -20° C: 14 days
Frozen -70° C: 30 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Grossly lipemic • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability • Samples submitted without two unique identifiers and date of collection


Methodology
Enzyme Linked Immunosorbent Assay (ELISA)

FDA Status
The performance characteristics of the listed assay was validated by BioAgilytix Diagnostics. The US FDA has not approved or cleared this test. The results of this assay can be used for clinical diagnosis without FDA approval. BioAgilytix Diagnostics is a CLIA certified, CAP accredited laboratory for performing high complexity assays such as this one.

Setup Schedule
Set up: Varies; Report available: 4 business days


Reference Range
0-22062 pg/mL


Clinical Significance
Transforming Growth Factor (TGF) plays a crucial role in tissue regeneration, cell differentiation, embryonic development, and regulation of the immune system. Transforming growth factor beta is found in hematopoietic (blood-forming) tissue and initiates a signaling pathway that suppresses the early development of cancer cells. It enhances the deposition of extracellular matrix and may play potential role in wound healing and cirrhosis formation. Many cells synthesize TGF-b and almost all of them have specific receptors for this peptide.


Performing Laboratory
BioAgilytix Diagnostics
1320 Soldiers Field Rd.
Boston, MA 02135



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.