Influenza A and B Virus with Subtyping, Real-Time PCR

Test Code

91335

CPT Codes
87502, 87503 (x3)

Includes

Influenza A RNA, QL PCR
Influenza B RNA, QL PCR
Influenza A H3
Influenza A H3N2V
2009 H1N1 Influenza RNA

Preferred Specimen

Nasal or nasopharyngeal swab submitted in 3 mL M4 media or VCM medium (green-cap) tube, or UTM equivalent

Minimum Volume

0.6 mL

Other Acceptable Specimens

3 mL nasopharyngeal aspirate collected in a sterile, leak-proof container

Instructions

Swabs in VCM, M4 or UTM transport media: Use only sterile Dacron or Rayon swabs. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator stick off near the tip to permit tightening of the cap.

Nasopharyngeal aspirate: Submit in a sterile container with a leak-proof cap.

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Calcium alginate swabs

Methodology
Real-Time Polymerase Chain Reaction • Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Sat; Report available: 2-3 days

Reference Range

Influenza A RNA, QL PCR	Not detected
Influenza B RNA, QL PCR	Not detected
Influenza A H3	Not detected
Influenza A H3N2V	Not detected
2009 H1n1 Influenza RNA	Not detected

Clinical Significance

This test is used to determine the presence of Influenza A or B viral RNA in a patient's specimen, and to differentiate among possible Influenza A virus subtypes.

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.