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Ehrlichia chaffeensis DNA, Real-Time PCR
Test Code11353
CPT Codes
87484
Preferred Specimen
0.7 mL whole blood collected in an EDTA (lavender-top), or acid citrate dextrose ACD (yellow-top) tube, or
1 tick (in 70% ethanol) submitted in a sterile, leak-proof container
1 tick (in 70% ethanol) submitted in a sterile, leak-proof container
Minimum Volume
Whole blood and CSF: 0.3 mL
Other Acceptable Specimens
1 mL CSF submitted in a sterile, leak-proof container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lithium heparin (green-top) tube • Sodium heparin (green-top) tube
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 1-3 days
Reference Range
Not detected
Clinical Significance
Ehrlichia chaffeensis DNA PCR is a highly sensitive method to detect the presence of ehrlichia chaffeensis DNA in clinical specimens.
The diagnosis of ehrlichia chaffeensis infection should not rely solely upon the result of PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of ehrlichia chaffeensis DNA at detectable levels in the sample tested and does not exclude the diagnosis of disease.
The diagnosis of ehrlichia chaffeensis infection should not rely solely upon the result of PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of ehrlichia chaffeensis DNA at detectable levels in the sample tested and does not exclude the diagnosis of disease.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
