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Parvovirus B19 DNA, Qualitative Real-Time PCR [34296X]
Test Code5319
CPT Codes
87798
Preferred Specimen
1 mL plasma collected in EDTA (lavender-top), ACD (yellow-top) or PPT potassium EDTA (white-top) tube or
1 mL whole blood collected in EDTA (lavender-top) or ACD (yellow-top) tube or
1 mL amniotic fluid or amniotic fluid supernatant collected in a sterile, leak-proof container or
1 mL serum or
> 3 cubic mm tissue collected in a sterile, plastic leak-proof container
1 mL whole blood collected in EDTA (lavender-top) or ACD (yellow-top) tube or
1 mL amniotic fluid or amniotic fluid supernatant collected in a sterile, leak-proof container or
1 mL serum or
> 3 cubic mm tissue collected in a sterile, plastic leak-proof container
Minimum Volume
>3 cubic mm fresh tissue
0.3 mL all other specimens
0.3 mL all other specimens
Transport Temperature
Whole blood: Refrigerated (cold packs)
All other samples: Frozen
All other samples: Frozen
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed whole blood • Heparin plasma or whole blood
Methodology
Real-Time Polymerase Chain Reaction (PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1-2 days
Reference Range
Not detected
Clinical Significance
Parvovirus B19 is the cause of an infection known as "Fifth Disease". This infection affects children primarily and causes a rash on the face, trunk, and limbs. Joint pain and swelling is more common in adults. Although a portion of those affected have only mild disease, patients with sickle cell anemia, or similar types of chronic anemia, can suffer from severe acute anemia. Infection during pregnancy can lead to severe complications for the fetus. Infections in immunocompromised patients may lead to serious complications including nephropathy, myocarditis, and persistent anemia. Although serologic detection of circulating antibodies (Parvovirus B-19 IgM & IgG) is useful to determine recent exposure to the virus, DNA testing provides the most reliable evidence of a persistent infection. Quantitative results of Parvovirus B19 viral load may be useful in determining the response to therapy (usually IVIG) and may be beneficial in assessing the risk for nosocomial transmission.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
