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HLA-A*02:01 Determination
Test Code92044
CPT Codes
81381
Includes
Samples positive for A*0201 require HLA A-High resolution testing for an additional fee.
Preferred Specimen
14 mL whole blood collected in an EDTA (lavender-top) tube
Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Minimum Volume
5 mL
Other Acceptable Specimens
Whole blood collected in: ACD (yellow-top), sodium or lithium (green-top) tube • 4 buccal swabs
Instructions
Sample cannot be shipped on Saturday or the day before a holiday.
Other acceptable sample types: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact Versiti Wisconsin for collection, transport and stability instructions.
Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.
Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Other acceptable sample types: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact Versiti Wisconsin for collection, transport and stability instructions.
Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.
Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Transport Temperature
Room temperature
Specimen Stability
Whole blood
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received room temperature >14 days • Received refrigerated • Received frozen
Methodology
Polymerase Chain Reaction • Direct DNA Sequencing
Setup Schedule
Set up: Mon-Sat; Report available: 7-9 days
Reference Range
See Laboratory Report
Performing Laboratory
Versiti Wisconsin, Inc
638 N 18 St
Milwaukee, WI 53233-2121
