Influenza A and B Antigen, DFA

Test Code
8357


CPT Codes
87275, 87276

Preferred Specimen
3 mL nasopharyngeal aspirate/wash, bronchial lavage/wash, in sterile leak-proof container or in VCM tube or FDA approved equivalent viral transport media, or
1 throat or nasopharyngeal swab in 3 mL VCM tube or FDA approved equivalent viral transport media


Minimum Volume
1 mL


Instructions
To maintain optimum viability, place swab or fluid into V-C-M (equal volumes of fluid and V-C-M) or equivalent and transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8°C or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens in V-C-M or equivalent should be frozen at -70°C or colder and transported on dry ice. Storage or transport at -20°C is not acceptable. Raw (unpreserved) specimens should only be refrigerated and not frozen.


Transport Temperature
Refrigerated (cold packs)


Specimen Stability

Raw (unpreserved) specimens
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable

VCM tube or FDA approved equivalent
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days



Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Throat specimens on <2 year old patients • Specimen in non-viral transport medium such as nucleic acid or bacterial transport media • Non-respiratory specimens • Dry swabs • Specimen in formalin or other fixatives • Received room temperature or frozen -20°C • Specimen received on slide


Methodology
Direct Immunofluorescence Assay (DFA)

Setup Schedule
Set up: Mon-Sat; Report available: 2-3 days


Reference Range
See Laboratory Report


Clinical Significance
Influenza type A and B viruses cause seasonal outbreaks of "the flu". Each winter, approximately 10-20% of the population are infected. Both type A and B are included in the flu vaccine.


Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.