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MSH2, IHC without Interpretation
Test Code16971
CPT Codes
88342
Preferred Specimen
Formalin-fixed, paraffin-embedded tissue block
Other Acceptable Specimens
Minimum: 5 unstained, positively charged tissue slides
Instructions
Do not freeze.
Transport Container
IHC Specimen Transport Kit
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Unacceptable
Refrigerated: Indefinitely
Frozen: Unacceptable
Methodology
Immunohistochemistry (IHC)
Setup Schedule
Set up: Mon-Fri; Report available: 2 days
Clinical Significance
Human mistmatch repair protein 2 (MSH2) is involved in
the initial recognition of mismatched nucleotides during
the postreplication mismatch repair process. Therefore,
the loss of MSH2 function leads to the accumulation of
replication errors, which in turn may be responsible for
the multiple mutations required for multistage
carcinogenesis. Mutations in mismatch repair genes have
been linked to hereditary nonpolyposis colon cancer and
to sporadic cancers that exhibit microsatellite
instability. MSH2 is reported to be expressed in the
nuclei of cells from a variety of tissues include
thyroid, heart, smooth muscle, and the germinal centers
of lymphoid follicles. In ileum and colon, MSH2
expression has been reported in the crypts, the cells of
which are undergoing rapid renewal. They are responsible
for the continuous production of differentiated cells
which migrate over 2 to 4 days before being sloughed into
the lumen.
the initial recognition of mismatched nucleotides during
the postreplication mismatch repair process. Therefore,
the loss of MSH2 function leads to the accumulation of
replication errors, which in turn may be responsible for
the multiple mutations required for multistage
carcinogenesis. Mutations in mismatch repair genes have
been linked to hereditary nonpolyposis colon cancer and
to sporadic cancers that exhibit microsatellite
instability. MSH2 is reported to be expressed in the
nuclei of cells from a variety of tissues include
thyroid, heart, smooth muscle, and the germinal centers
of lymphoid follicles. In ileum and colon, MSH2
expression has been reported in the crypts, the cells of
which are undergoing rapid renewal. They are responsible
for the continuous production of differentiated cells
which migrate over 2 to 4 days before being sloughed into
the lumen.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
