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Culture, Urine, Routine [395X]
Test Code665
CPT Codes
87086
Includes
If culture is positive, CPT code(s): 87088 (each isolate) will be added with an additional charge.
Identification will be performed at an additional charge (CPT code(s): 87077 or 87140 or 87143 or 87147 or 87149).
Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87181 or 87184 or 87185 or 87186).
Identification will be performed at an additional charge (CPT code(s): 87077 or 87140 or 87143 or 87147 or 87149).
Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87181 or 87184 or 87185 or 87186).
Preferred Specimen
4 mL random, clean catch, midstream urine collection in gray-top urine transport tube or UriSponge™ transport
Minimum Volume
Preserved: gray-top transport must be filled to minimum line printed on tube • Completely saturated UriSponge transport • Unpreserved: 0.5 mL urine
Other Acceptable Specimens
Indwelling catheter collected urine • Unpreserved urine specimens <3 mL or from pediatric, patients with renal abnormalities, or difficult collections are acceptable when placed in a sterile leak-proof container
Instructions
Gray-top tube: Fill to the fill line (4 mL) indicated on the tube label.
UriSponge transport: Dip sponge applicator into the urine sample and submerge for 5 seconds. Remove applicator from the urine sample and return it to UriSponge transport, securing tightly.
UriSponge transport: Dip sponge applicator into the urine sample and submerge for 5 seconds. Remove applicator from the urine sample and return it to UriSponge transport, securing tightly.
Transport Temperature
Preserved: Room temperature
Unpreserved: Refrigerated (cold packs) only
Unpreserved: Refrigerated (cold packs) only
Specimen Stability
Preserved
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Unpreserved
Room temperature: Unacceptable
Refrigerated: 24 hours
Frozen: Unacceptable
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Unpreserved
Room temperature: Unacceptable
Refrigerated: 24 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Expired transport devices • Leaking specimens • Specimens submitted in transports other than those listed as acceptable • Unpreserved urine >3 mL • Urine tube not filled to minimum fill line • Foley tips • Specimens collected from catheter bag • 24-hour urine collections • Uricult devices • Inadequately saturated UriSponge • UriSponge transport filled with urine
Methodology
Culture
Setup Schedule
Set up: Daily; Report available: 3 days
Reference Range
No growth
Clinical Significance
Urinary tract infections (UTI) are among the most common human infections. Infections may involve the urethra, bladder, kidneys, other organs, or adjacent tissues and may be complicated by bacteremia. The predictors of UTI are pyuria and bacteriuria. Microscopy and other rapid detection systems can detect pyuria. In voiding, the urine must pass through sites containing normal flora. Quantitative culturing of urine is an established tool to differentiate significant bacteriuria from contamination introduced during voiding. However, studies have shown that when the calibrated method is performed, even perfectly, colony counts are only approximations.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

