Amikacin, Peak and Trough

Test Code

250

CPT Codes
80150 (x2)

Preferred Specimen

1 mL serum collected in each of two red-top tubes (no gel)

Patient Preparation
Collect peak at end of a 60 minute IV infusion, or 30 minutes after end of 30 minute infusion, or 60 minutes after IM dose.

Collect as a trough just prior to next dose.

Minimum Volume

0.2 mL

Other Acceptable Specimens

1 mL plasma collected in each of two EDTA (lavender-top) tubes, or fluoride oxalate (gray-top) tubes

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Serum separator tube • Heparin plasma

Methodology
Immunoassay (IA)

Setup Schedule

Set up: Mon-Sat; Report available: 1-2 days

Reference Range

Amikacin, Peak	20.0-30.0 mg/L
Amikacin, Trough	4.0-8.0 mg/L

Clinical Significance

Amikacin is an aminoglycoside antibiotic used in the treatment of gram-negative bacillary infections. Amikacin is potentially nephrotoxic and ototoxic and serial monitoring of serum levels provides information to maintain safe and therapeutic levels.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.