Prothrombin Antibody (IgG)

94041

1 mL plasma collected in a 3.2% sodium citrate (light blue-top) tube

0.3 mL

Serum

Citrated plasma (preferred): Blood should be collected by venipuncture, and the plasma separated from the cells immediately by centrifugation at 1500 x g for 10 minutes.

Serum (acceptable): Blood should be collected by venipuncture, and the serum separated from the cells by centrifugation after clot formation.

Supernatant fluid must be carefully removed after centrifugation to avoid contamination with platelets. Repeating the centrifugation and separation steps may be advisable to minimize platelet contamination. Transport frozen samples on dry ice.

Frozen

Room temperature: 48 hours
Refrigerated: 14 days
Frozen: 21 days

Gross hemolysis • Received room temperature

Set up: Once a week; Report available: 2 days

<20 G units

This test is intended for the detection and semi-quantitation of IgG anti-prothrombin (aPT) antibodies. Antiphospholipid antibodies are a heterogenous group of antibodies that bind to protein-phospholipid complexes. aPT antibodies are included in this category and may be detected in patients with autoimmune diseases (e.g. systemic lupus erythematosus) and/or with antiphospholipid syndrome (APS). The aPT antibody concentration values obtained from this assay are an aid to diagnosis only. If the results from this test are negative, and the clinical history is suggestive of APS, suggest testing for the APS criteria antibodies - IgG and IgM beta-2-glycoprotein I and cardiolipin antibodies, and lupus anticoagulant. If the latter testing is positive, it is recommended to repeat testing in at least 12 weeks to demonstrate persistence of antibodies.