Protein S Activity with Reflex to Protein S Antigen, Total and Free [17494X]

Test Code

17494

CPT Codes
85306

Includes

If Protein S Activity is <60% for females or <70% for males, then Protein S Antigen Total and Free will be performed at an additional charge (CPT code(s): 85305, 85306).

Preferred Specimen

2 mL frozen platelet-poor plasma collected in 3.2% sodium citrate (light blue-top) tube

Minimum Volume

1 mL

Instructions

Please submit a separate, frozen vial for each special coagulation assay ordered.

Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Plasma must be free of platelets (<10,000/mcL). Freeze immediately and transport on dry ice.

Transport Container

Plastic screw-cap vial

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis

Methodology
Clot Detection • Immunotrubidimetric

Setup Schedule

Set up: Tues-Sat; Report available: 1-3 days

Reference Range

Protein S Activity

Males	70-150	%normal
Female	60-140	%normal

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.