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Factor IX Activity and Human Inhibitor
Test Code17845
CPT Codes
85250, 85335
Preferred Specimen
2 mL (x2) frozen platelet-poor plasma collected in two 3.2% sodium citrate (light blue-top) tube
Minimum Volume
1 mL (x2)
Instructions
Platelet poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (< 10,000/mcL). Freeze immediately and ship on dry ice. Do not thaw.
Transport Container
Plastic screw-cap vial (x2)
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 14 days
Refrigerated: Unacceptable
Frozen: 14 days
Methodology
Clot Based Assay • Photometric Clot Detection
Setup Schedule
Set up: Tues; Report available: 3-10 days
Reference Range
| Factor IX Activity | 60-160 | % normal |
| Factor IX Inhibitor | ≤0.4 | BU |
Clinical Significance
Factor IX Human Inhibitor provides a quantitative determination of inhibitors. This aids in developing treatment strategies for patients with hemophilia.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
