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Penicilloyl V (C2) IgE [703X]
Test Code70389
CPT Codes
86003
Preferred Specimen
0.3 mL serum
Minimum Volume
0.15 mL
Instructions
Collect blood in a red-top (no gel) or serum separator tube (gel). Allow blood to clot (10-15 minutes) at room temperature. Centrifuge and separate the serum from the cells and transfer serum to a clean transport tube.
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Methodology
Immunoassay (IA)
Setup Schedule
Set up: Mon-Sat; Report available: 1-3 days
Reference Range
See Laboratory Report
Clinical Significance
This procedure is based on a fluorescent enzyme immunoassay (FEIA) technique. The allergen of interest, covalently coupled to an ImmunoCap, reacts with the specific IgE in the patient?s serum. After washing away nonspecific IgE, enzyme-labelled antibodies against IgE are added to form a complex. After incubation, unbound enzyme anti-IgE is washed away and the bound complex is then incubated with a developing agent. After the reaction is stopped, the fluorescence of the eluate is measured.
The higher the fluorescence value, the more specific IgE is present in the specimen. To classify test results, fluorescence for patient samples is compared directly with fluorescence for a reference serum run in parallel. Results are reported in both kU/L and Conventional Class score.
The higher the fluorescence value, the more specific IgE is present in the specimen. To classify test results, fluorescence for patient samples is compared directly with fluorescence for a reference serum run in parallel. Results are reported in both kU/L and Conventional Class score.
Performing Laboratory
Quest Diagnostics Nichols Institute |
14225 Newbrook Drive |
Chantilly, VA 20153 |