A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Nicotine and Expanded Metabolites, LC/MS/MS, Urine
Test Code90982
CPT Codes
80323
Includes
Nicotine, Cotinine, 3-OH-Cotinine, Nornicotine, Norcotinine, Anabasine
Preferred Specimen
1 mL urine
Minimum Volume
0.5 mL
Transport Container
Plastic screw-cap vial or Plastic urine container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Methodology
Chromatography/Mass Spectrometry
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat evening; Report available: 3 days
Reference Range
Non-Smoker (ng/mL) | Active Tobacco User (ng/mL) | |
Nicotine | <17 | 200-700 |
Cotinine | <20 | 300-1300 |
3-OH-Cotinine | <50 | 3000-25000 |
Nornicotine | <2 | 30-900 |
Norcotinine | <2 | Not established |
Anabasine | <2 | 10-500 |
Clinical Significance
This assay is used for the detection of nicotine and several metabolites and the related alkaloid anabasine in urine to determine the tobacco exposure status of the individual. Nicotine has a short half-life of approximately sixty minutes; Its presence may indicate recent tobacco exposure. The half-life of the various nicotine metabolites is longer and provides a larger window of detection for tobacco exposure. The presence of anabasine is indicative of tobacco exposure (smoked or smokeless) versus the use of a nicotine replacement patch product. Anabasine is a related alkaloid found only in the tobacco plant. Individuals exposed to passive tobacco smoke (environmental or second-hand) can have detectable concentrations of nicotine and its metabolites depending on the type of exposure. The ranges of concentrations related to passive exposure are not well established but normally are less than those attributed to active tobacco users.
Performing Laboratory
Quest Diagnostics Nichols Institute |
14225 Newbrook Drive |
Chantilly, VA 20153 |