AccuType® IL28B (NY)

Test Code

90252

CPT Codes
81283<br /> This test is for New York State clients only. For Non-New York State patient testing, see Test Code 90251.

Physician Attestation of Informed Consent

This germline genetic test requires physician attestation that patient consent has been received if ordering medical facility is located in AK, DE, FL, GA, IA, MA, MN, NV, NJ, NY, OR, SD or VT or test is performed in MA.

Preferred Specimen

5 mL whole blood collected in an EDTA (lavender-top) tube

Patient Preparation
Do not eat immediately prior to sample collection. If patient has eaten recently, rinse mouth with plain water.

Minimum Volume

2 mL

Other Acceptable Specimens

Whole blood collected in: ACD solution A or B (yellow-top) tube • Buccal swab collected in: DNA Genotek Oracollect Kit • Celera DNA Collection Kit (follow collection kit instructions)

Instructions

Do not freeze whole blood.

Buccal swab: Use only Celera/Copan DNA Collection Kit (Celera part # is 5002737) or DNA Genotek OraCollect Kit (OC-100). Follow collection kit instructions.

Transport Temperature

Room temperature

Specimen Stability

Whole blood
Room temperature: 8 days
Refrigerated: 8 days
Frozen: Unacceptable

Buccal swab
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received frozen • Buccal swab received refrigerated • Incorrect collection device • Liquid has leaked from container/no liquid present

Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)

Setup Schedule

Set up: Daily p.m.; Report available: 3 days

Reference Range

See Laboratory Report

Clinical Significance

The C polymorphism in rs12979860 is strongly associated with a two fold greater sustained virological response in European, African American, and Hispanic populations. Knowledge of the host genotype of patients infected with HCV will aid in the clinical decision to initiate treatment with PegIFN and RBV (a 48 week course of interferon and ribavirin which has limited efficacy and is often poorly tolerated due to side effects that prevent patients from finishing treatment).

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.