HIV-1 Genotype (RTI, PI, Integrase Inhibitors)

Test Code

91692

CPT Codes
87900, 87901, 87906

Includes

HIV-1 Integrase Genotype
HIV-1 Genotype

Preferred Specimen

4 mL plasma collected in an EDTA (lavender top) or PPT (white top) tube

Minimum Volume

1.2 mL

Other Acceptable Specimens

CSF collected in a sterile leak-proof container

Instructions

Do not freeze whole blood or any samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. If shipping frozen, transfer plasma to a leak-proof transport tube, then freeze prior to shipment. Follow manufacturer's instructions for collection tube handling.

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

Room temperature: 24 hours
Refrigerated: 6 days
Frozen: 42 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Plasma received frozen in the plasma preparation tube (PPT) • Serum • Whole blood greater than 24 hours old • Frozen whole blood

Methodology
Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) • Sequencing

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Sat; Report available: 4-9 days

Reference Range

See Laboratory Report

Clinical Significance

Identify drug resistance mutations in HIV-1 patients failing antiretroviral regimens containing RT, PR or Integrase inhibitors.
Identify transmitted drug resistance mutations in the RT, PR or integrase genes in treatment-naive patients prior to initiation of antiretroviral therapy.

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.