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HIV-1 RNA, Quantitative, Real-Time PCR with Reflex to Genotype (RTI, PI, Integrase)
Test Code91691
CPT Codes
87536
Includes
If HIV-1 RNA, Quantitative, Real-Time PCR viral load is >400 copies/mL, then HIV-1 Genotype and HIV-1 Integrase Genotype will be performed at an additional charge (CPT code(s): 87900, 87901, 87906).
Preferred Specimen
7 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
3.7 mL
Other Acceptable Specimens
Plasma collected in: PPT Potassium EDTA (white-top) tube
Instructions
Samples submitted in primary tube will be rejected.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Follow manufacturer's instructions for collection tube handling.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Follow manufacturer's instructions for collection tube handling.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 30 days
Refrigerated: 6 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples received in primary collection tube • Gross hemolysis • Lipemia
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Set up: Mon-Sat; Report available: 3-5 days
Reference Range
HIV-1 RNA, QN, PCR | Not detected | copies/mL | |
HIV-1 RNA, QN, PCR | Not detected | Log copies/mL |
Clinical Significance
Monitor viral load in HIV-1 patients undergoing antiretroviral therapy and automatically reflex to drug resistance testing for PR, RT and Integrase drugs in patients failing to achieve or maintain virologic suppression.
Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
33608 Ortega Highway |
San Juan Capistrano, CA 92675-2042 |